Coding

Part:BBa_K4821002

Designed by: Madeline Ho   Group: iGEM23_Washington   (2023-10-12)


RdhANp_WT_K12

This sequence codes for the RdhANp protein (PBD ID 6ZXX), a reductive dehalogenase found in Nitratireductor pacificus.The part is similar to part BBa_K2462000 by WHU-China, as that part also codes for wild-type RdhANp, but the codon sequences have been optimized for E. coli K-12.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1628
    Illegal BamHI site found at 312
    Illegal BamHI site found at 419
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None